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Transcriptomic Characterization of a Synergistic Genetic Interaction during Carpel Margin Meristem Development in Arabidopsis thaliana.
PLoS One. 2011;6(10):e26231
Authors: Wynn AN, Rueschhoff EE, Franks RG
Abstract
In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant double mutant suggests that SEU and ANT share overlapping functions during CMM development. However the molecular mechanism underlying this synergistic interaction is unknown. Using the ATH1 transcriptomics platform we identified transcripts that were differentially expressed in seu ant double mutant relative to wild type and single mutant gynoecia. In particular we sought to identify transcripts whose expression was dependent on the coordinated activities of the SEU and ANT gene products. Our analysis identifies a diverse set of transcripts that display altered expression in the seu ant double mutant tissues. The analysis of overrepresented Gene Ontology classifications suggests a preponderance of transcriptional regulators including multiple members of the REPRODUCTIVE MERISTEMS (REM) and GROWTH-REGULATING FACTOR (GRF) families are mis-regulated in the seu ant gynoecia. Our in situ hybridization analyses indicate that many of these genes are preferentially expressed within the developing CMM. This study is the first step toward a detailed description of the transcriptional regulatory hierarchies that control the development of the CMM and ovule initiation. Understanding the regulatory hierarchy controlled by SEU and ANT will clarify the molecular mechanism of the functional redundancy of these two genes and illuminate the developmental and molecular events required for CMM development and ovule initiation.
PMID: 22031826 [PubMed - in process]

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Duncan Craig2010 has added a photo to the pool:

Taken after the welcome beginning of our summer rains.
never miss a day, thanks for the blog

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so interesting

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wonder what this really means

Black Diamond Images posted a reply:

Ceiba insignus – North Coast Regional Botanic Gardens, Coffs Harbour, NSW, Australia

this is bogus, who would think

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how doe this impact my state

this is bogus, who would think

Nonsense-mediated mRNA decay factors, UPF1 and UPF3, contribute to plant defense.
Plant Cell Physiol. 2011 Oct 24;
Authors: Jeong HJ, Kim YJ, Kim SH, Kim YH, Lee IJ, Kim YK, Shin JS
Abstract
In Arabidopsis, the NMD-defective mutants, upf1-5 and upf3-1 are characterized by dwarfism, curly leaves and late flowering. These phenotypes are similar to those of mutants showing constitutive PR gene expression, SA accumulation and, subsequently, resistance to pathogens. The disease symptoms of upf1-5 and upf3-1 mutants were observed following infection with the virulent pathogen Pst DC3000 with the aim of determining whether the loss of NMD is involved in disease resistance. These mutant plants not only showed enhanced resistance to Pst DC3000, but also elevated levels of endogenous SA, PR gene transcripts, and WRKY transcripts. UPF1 and UPF3 expression was downregulated in Pst DC3000-infected Arabidopsis plants, but the expression of various NMD target genes was upregulated. The expression of ten defense-related genes was elevated in CHX-treated plants. The transcriptional ratios of eight of these ten defense-related genes in CHX-treated to non-treated plants were lower in NMD-defective mutants than the wild-type plants. These eight defense-related genes are possibly regulated by NMD mechanism, and it is clear that an alternatively spliced transcript of WRKY62, which contains a PTC, was regulated by this mechanism. Taken together, our results suggest that UPF1 and UPF3, which are key NMD factors, may act as defense-related regulators associated with plant immunity.
PMID: 22025558 [PubMed - as supplied by publisher]

and i though i was the only one to see it this way

BOILLON CHRISTOPHE has added a photo to the pool:

JUSTE UN PETIT PLAISIR POUR MES CONTACTS SUR FLICKR
des couleurs de crocus en vallée de chamonix
MACRO À VOIR EN GRANDE TAILLE SUR FOND NOIR JUSTE POUR LES COULEURS
www.photosboillonchristophe.com
I like this:)