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Archive for December, 2011
November 2008: Winter Wonderland
Thursday, December 29th, 2011Development of a transgenic early flowering pear (Pyrus communis L.) genotype by RNAi silencing of PcTFL1-1 and PcTFL1-2.
Thursday, December 29th, 2011 Development of a transgenic early flowering pear (Pyrus communis L.) genotype by RNAi silencing of PcTFL1-1 and PcTFL1-2.
Planta. 2011 Dec 28;
Authors: Freiman A, Shlizerman L, Golobovitch S, Yablovitz Z, Korchinsky R, Cohen Y, Samach A, Chevreau E, Le Roux PM, Patocchi A, Flaishman MA
Abstract
Trees require a long maturation period, known as juvenile phase, before they can reproduce, complicating their genetic improvement as compared to annual plants. ‘Spadona’, one of the most important European pear (Pyrus communis L.) cultivars grown in Israel, has a very long juvenile period, up to 14Â years, making breeding programs extremely slow. Progress in understanding the molecular basis of the transition to flowering has revealed genes that accelerate reproductive development when ectopically expressed in transgenic plants. A transgenic line of ‘Spadona’, named Early Flowering-Spadona (EF-Spa), was produced using a MdTFL1 RNAi cassette targeting the native pear genes PcTFL1-1 and PcTFL1-2. The transgenic line had three T-DNA insertions, one assigned to chromosome 2 and two to chromosome 14 PcTFL1-1 and PcTFL1-2 were completely silenced, and EF-Spa displayed an early flowering phenotype: flowers developed already in tissue culture and on most rooted plants 1-8Â months after transfer to the greenhouse. EF-Spa developed solitary flowers from apical or lateral buds, reducing vegetative growth vigor. Pollination of EF-Spa trees generated normal-shaped fruits with viable F1 seeds. The greenhouse-grown transgenic F1 seedlings formed shoots and produced flowers 1-33Â months after germination. Sequence analyses, of the non-transgenic F1 seedlings, demonstrated that this approach can be used to recover seedlings that have no trace of the T-DNA. Thus, the early flowering transgenic line EF-Spa obtained by PcTFL1 silencing provides an interesting tool to accelerate pear breeding.
PMID: 22203321 [PubMed - as supplied by publisher]
maybe this will change things
flyrose
Thursday, December 29th, 2011hynes.jane has added a photo to the pool:
more news brought to your by our blog, thanks for visiting
Climbing Roses
Thursday, December 29th, 2011Climbing roses are one of the gardeners’ favorite types of roses. They add grace and nostalgia and tenderness to any home garden. Climbing roses are often vigorous plants that will reach varying heights depending on the cultivar. All climbing rose species have stiff, arching stems, usually with dense, glossy leaves divided into small leaflets. The [...]
this is bogus, who would think
Lenox Rutledge Gold Banded Ivory China 20-Piece Dinnerware Set, Service for 4 (Kitchen) newly tagged “flowered”
Thursday, December 29th, 2011 Lenox Rutledge Gold Banded Ivory China 20-Piece Dinnerware Set, Service for 4 (Kitchen)By Lenox Buy new: $599.95 Customer Rating: First tagged “flowered” by P. Newton “AZ Minis” Customer tags: lenox, flowered, cream, dinnerware, china, fine china
does anyone know when this will take effect
Reply to FOLIAGE, FRUITS, SEEDS, TRUNKS, BARK, etc.
Thursday, December 29th, 2011Tatters:) posted a reply:
we know that fruit of the tree can be more attractive than its flowers.
Spindle-tree is an example:
Euonymus europaeus seedpods:
does anyone know when this will take effect
50% off all-clad roasting set with rack – Cooking.com
Thursday, December 29th, 2011Get 50% off all-clad roasting set with rack at Cooking.com.
more to read in our archives
November 2008: Winter Wonderland
Monday, December 26th, 2011thats funny
GmFT2a, a Soybean Homolog of FLOWERING LOCUS T, Is Involved in Flowering Transition and Maintenance.
Monday, December 26th, 2011 GmFT2a, a Soybean Homolog of FLOWERING LOCUS T, Is Involved in Flowering Transition and Maintenance.
PLoS One. 2011;6(12):e29238
Authors: Sun H, Jia Z, Cao D, Jiang B, Wu C, Hou W, Liu Y, Fei Z, Zhao D, Han T
Abstract
BACKGROUND: Flowering reversion can be induced in soybean (Glycine max L. Merr.), a typical short-day (SD) dicot, by switching from SD to long-day (LD) photoperiods. This process may involve florigen, putatively encoded by FLOWERING LOCUS T (FT) in Arabidopsis thaliana. However, little is known about the potential function of soybean FT homologs in flowering reversion.
METHODS: A photoperiod-responsive FT homologue GmFT (renamed as GmFT2a hereafter) was cloned from the photoperiod-sensitive cultivar Zigongdongdou. GmFT2a gene expression under different photoperiods was analyzed by real-time quantitative PCR. In situ hybridization showed direct evidence for its expression during flowering-related processes. GmFT2a was shown to promote flowering using transgenic studies in Arabidopsis and soybean. The effects of photoperiod and temperature on GmFT2a expression were also analyzed in two cultivars with different photoperiod-sensitivities.
RESULTS: GmFT2a expression is regulated by photoperiod. Analyses of GmFT2a transcripts revealed a strong correlation between GmFT2a expression and flowering maintenance. GmFT2a transcripts were observed continuously within the vascular tissue up to the shoot apex during flowering. By contrast, transcripts decreased to undetectable levels during flowering reversion. In grafting experiments, the early-flowering, photoperiod-insensitive stock Heihe27 promotes the appearance of GmFT2a transcripts in the shoot apex of scion Zigongdongdou under noninductive LD conditions. The photothermal effects of GmFT2a expression diversity in cultivars with different photoperiod-sensitivities and a hypothesis is proposed.
CONCLUSION: GmFT2a expression is associated with flowering induction and maintenance. Therefore, GmFT2a is a potential target gene for soybean breeding, with the aim of increasing geographic adaptation of this crop.
PMID: 22195028 [PubMed - in process]
does anyone know when this will take effect
Golden Cups
Monday, December 26th, 2011IanAWood has added a photo to the pool:
it should be interested what change will this make